| 质粒名称: | pLgw V5-EcoDam |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 59210 |
| 存储实验室: | Bas van Steensel |
|
载体骨架基本信息 |
|
| 载体名称: | HIV-CS-CG |
| 载体出品公司: | PMID 9733856 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒；DamID |
| 筛选标记: | Zeo标记在LTRs之外，不会被包装成病毒。 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EcoDam,DNA adenine methyltransferase |
| 物种来源: | E. coli |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | heat shock minimal pomoter |
| 标签/融合蛋白: | V5（主干上的N终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pCasper-hs |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLgw V5-EcoDam was a gift from Bas van Steensel (Addgene plasmid # 59210 ; http://n2t.net/addgene:59210 ; RRID:Addgene_59210) |
| 参考文献部分: | Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, Feenstra M, Abbas B, Classen AK, van Steensel B. Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12. 10.1101/gr.5391806 PubMed 17038565 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLgw V5-EcoDam 8629 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Mammalian DamID control lentiviral vector for expression of V5-Dam
only .
ACCESSION .
VERSION .
KEYWORDS pLgw V5-EcoDam
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8629)
AUTHORS Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W,
Feenstra M, Abbas B, Classen AK, van Steensel B
TITLE Human heterochromatin proteins form large domains containing
KRAB-ZNF genes.
JOURNAL Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
PUBMED 17038565
REFERENCE 2 (bases 1 to 8629)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8629
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 36..171
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 98..117
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 233..280
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 299..673
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 803..924
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(840..859)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 894..913
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(973..989)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(973..989)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(986..1008)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 997..1013
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1021..1051)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1066..1087
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1204..1221)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1375..1963)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1455..1474)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2134..2994)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2757..2776
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2995..3099)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3174..3193)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3365..3744
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3746..3944
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3899..3919
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 3962..4142
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4186..4311
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 4804..5037
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 5728..5966
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind 5913..5934
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
protein_bind 6019..6043
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(6119..6143)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 6154..6195
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
LTR 7560..7740
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7766..7783)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7772..7996
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8042..8470
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8129..8148)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8339..8360
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8479..8499)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8484..185
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
ORIGIN
1 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
61 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
121 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
181 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
241 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
301 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
361 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
421 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
481 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
541 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
601 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
661 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
1021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
1081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
1141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
1201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
1261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
1381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
1441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
1501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
1561 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
1621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
1681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
1741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
1801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
1861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
1921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
1981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3121 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
3181 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
3241 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
3301 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
3361 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
3421 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
3481 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
3541 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
3601 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
3661 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
3721 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
3781 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
3841 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
3901 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
3961 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
4021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
4081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
4141 cagtggcgcc cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg
4201 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
4261 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
4321 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
4381 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
4441 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
4501 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
4561 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
4621 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
4681 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
4741 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
4801 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc
4861 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
4921 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
4981 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
5041 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
5101 gagtaataaa tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga
5161 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
5221 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
5281 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
5341 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
5401 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
5461 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcccccgg
5521 gctgcaggaa ttcagatctc ggccgcatat taagtgcatt gttctcgata ccgctaagtg
5581 cattgttctc gttagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac
5641 aagtgcattg ttctcttgct gaaagctcga tggacaagtg cattgttctc ttgctgaaag
5701 ctcagtaccc gggagtaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga
5761 gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata
5821 aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa
5881 ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa
5941 ttattgaata caagaagaga actctgaata ctttcaacaa gttaccgaga aagaagaact
6001 cacacacagc tagatcaaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
6061 accatggaaa agaatggaac tgatgaagta tatattttta ataagggtgg gcgcgccgac
6121 ccagctttct tgtacaaagt ggttcgatcc ggtggtaagc ctatccctaa ccctctcctc
6181 ggtctcgatt ctacgatcga tttcgaaccg cggatgaaga aaaatcgcgc ttttttgaag
6241 tgggcagggg gcaagtatcc cctgcttgat gatattaaac ggcatttgcc caagggcgaa
6301 tgtctggttg agccttttgt aggtgccggg tcggtgtttc tcaacaccga cttttctcgt
6361 tatatccttg ccgatatcaa tagcgacctg atcagtctct ataacattgt gaagatgcgt
6421 actgatgagt acgtacaggc cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag
6481 gtttactatc agttccgcga agagttcaac aaaagccagg atccgttccg tcgggcggta
6541 ctgtttttat atttgaaccg ctacggttac aacggcctgt gtcgttacaa tctgcgcggt
6601 gagtttaacg tgccgttcgg ccgctacaaa aaaccctatt tcccggaagc agagttgtat
6661 cacttcgctg aaaaagcgca gaatgccttt ttctattgtg agtcttacgc cgatagcatg
6721 gcgcgcgcag atgatgcatc cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg
6781 accgccaact ttacggcgta tcacacaaac agttttacgc ttgaacaaca agcgcatctg
6841 gcggagatcg ccgaaggtct ggttgagcgc catattccag tgctgatctc caatcacgat
6901 acgatgttaa cgcgtgagtg gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc
6961 agtataagca gcaacggcgg cacacgtaaa aaggtggacg aactgctggc tttgtacaaa
7021 ccaggagtcg tttcacccgc gaaaaaataa ctcgagacct agaaaaacat ggagcaatca
7081 caagtagcaa tacagcagct accaatgctg cttgtgcctg gctagaagca caagaggagg
7141 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
7201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
7261 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
7321 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
7381 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
7441 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
7501 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
7561 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
7621 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
7681 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
7741 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
7801 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
7861 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
7921 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
7981 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
8041 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
8101 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
8161 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
8221 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
8281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
8341 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
8401 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
8461 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
8521 caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
8581 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatt
//
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